Digital PCR and Real-time PCR (qPCR) involves creating a sequence of polymers using digital probes and temperature gradients. This is followed by denaturation in which multiple restriction enzymes are introduced to generate the final product. The usage of Digital Performing Polymerase Chain Reaction (DPCR) and Real-time Polymerase Chain Reaction (RTPCR) has wide applications in the field of genomics. Digital PCR and Real-time PCR can also be applied in different settings like the pre-tissue culture of cells, growth of various types of organisms, forensic examination of biological samples, etc.
The usage of DNA profiling has
evolved through the last few years with the advent of digital sampling methods.
The Digital Polymerase Chain Reaction (DPCR) and the Real-Time Polymerase Chain
Reaction (RTP) are two popular DNA profiling methods. DPCR method works on
single molecules whereas the RTP method works on multiple strands of DNA
simultaneously. The usage of both these techniques greatly helps in the
detection of the variation in the genetic material present in an individual.
The increasing demand for Digital
PCR and Real-time PCR (qPCR) Market and systems in a variety of
settings such as healthcare, industrial, academic, and government require
rapid and reliable experimental designs and data gathering capabilities.
Digital PCR and Real-time PCR experiments are ideally suited for in silico
experiments due to their speed and low data requirements. In silico
experiments, researchers can easily run on single samples and evaluate their
properties using single experiments. As compared to traditional DNA
experiments, or and qpcr experiments are highly flexible and can be designed
according to the experimental requirements of the researcher. For instance,
there is no restriction on the types of experiments that can be performed using
Digital PCR and Real-time PCR (qPCR). Other applications of Digital PCR and
Real-time PCR are for the purpose of forensic analysis.