Chemiluminescence
imaging is an important part of molecular biology research. The process allows
researchers to visualize a chemical reaction in its most natural form. These
images can be saved on discs and used as a reference for further studies.
However, there are many limitations to this technique. For example, it does not
provide information about the chiral composition of the sample. This makes it
unsuitable for high-throughput screening.
Besides
detecting metabolites, chemiluminescence imaging can also detect enzyme activity
and localize the activity of a cell's cytosolic proteins. This technique is
more accurate than fluorescence microscopy, but the resolution is lower. In
some cases, it is used to detect metabolites in shock-frozen tissue biopsies.
In this method, the tissue is frozen to stop enzyme activity. Then, it is
inserted into a microscope stage at a temperature-controlled temperature and
exposed to a chemiluminescence reagent.
This
technology targets DNA sequences, antigens, enzymes, and metabolites and is highly
sensitive. It is used to study the chemical processes in tissues and cells. In
some cases, it can even be used to study whole animals. There are a few
limitations to this method, but it is widely used in many research studies.
These challenges make it a viable option for researchers. The most common
challenges associated with chemiluminescence imaging are related to
sensitivity.
Furthermore,
research on the global Chemiluminescence Imaging market gives a critical
evaluation of the user experience to aid management decision-making in
establishing a successful plan for obtaining more clients.
The
main challenges of chemiluminescence imaging
are time, quality of the data, and sensitivity. The detection of a protein of
interest depends on the time required for the enzyme to consume its substrate.
Therefore, the blot must be imaged before the substrate is consumed or the
light signal decays. Exposure time should increase as the signal decreases over
several minutes. During long experiments, researchers may choose multiple exposures
to optimize their results.
Players
in the chemiluminescence imaging market are focused on launching new products.
In June 2020, Syngene, a U.K-based manufacturer of image analysis solutions,
announced that it’s GeneSys software is available with the addition of new
ChemiRapid, Signal Accumulation Calculator, and Enhanced Image features that
enhance chemiluminescence imaging workflow and quality.